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Schmittgen, J. Jiang, Q. Liu and L. Yang Nucleic Acids Res. The present study adapted the assay to a well format and expanded it to include primers to human miRNA precursors. TaqMan minor groove binder probes were used to discriminate nearly identical members of the let-7 family of miRNA isoforms.
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Adipocytes can dedifferentiate into fibroblast-like cells in vitro and thereby acquire proliferation and multipotent capacities to participate in the repair of various organs and tissues. Whether dedifferentiation occurs under physiological or pathological conditions in vivo is unknown. A tissue expander was placed under the inguinal fat pads of rats and gradually expanded by injection of water. Samples were collected at various time points, and morphological, histological, cytological, ultrastructural, and gene expression analyses were conducted. The transplanted adipocytes were assessed by bioluminescence imaging and whole-mount staining. The expanded fat pad was obviously thinner than the untreated fat pad on the opposite side.
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He can be reached at: stillnc wildblue. We publish his article about mulberries as our July 4 th gift to our readers. For those of us of a certain age, mulberries and childhood went hand in hand. I was an adult before I learned that actual mulberry trees were all around us as well. We have two species, one red and one white, one native and one introduced.
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Slime mold or slime mould is an informal name given to several kinds of unrelated eukaryotic organisms that can live freely as single cells, but can aggregate together to form multicellular reproductive structures. Slime molds were formerly classified as fungi but are no longer considered part of that kingdom. More than species of slime mold occur all over the world. Their common name refers to part of some of these organisms' life cycles where they can appear as gelatinous "slime". This is mostly seen with the myxogastria , which are the only macroscopic slime molds.
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